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gradient denaturing sdspage gel (Bio-Rad)

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Bio-Rad gradient denaturing sdspage gel
Gradient Denaturing Sdspage Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 13223 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gradient+sdspage+gels/MINI-PROTEAN+TGX/pm41484378-213-18-24
Average 99 stars, based on 13223 article reviews

gradient denaturing sdspage gel - by Bioz Stars, 2026-09

99/100 stars

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Article Title: Proinflammatory Cytokine Interleukin 1β Disrupts β-cell Circadian Clock Function and Regulation of Insulin Secretion.
Article Snippet: Protein lysates were ran on Mini-Protean TGX 4–20% gradient SDSPAGE gels (BioRad) and transferred to 0.2 μm PVDF Trans-blot Turbo membranes (BioRad) using the Trans-blot Turbo System (BioRad).

Activity Assay:

Article Title: DNA polymerase gamma mutations that impair holoenzyme stability cause catalytic subunit depletion.
Article Snippet: Samples were incubated at 37 ◦C for 0–90 min in a buffer containing 50 mM Tris–HCl pH 8.0, 10 mM MgCl2, 0.1 mg/ml BSA, 2 mM ATP and 1 mM DTT and the reactions were stopped by addition of Laemli sample buffer (BioRad). .. Samples were run on precast 4–20% gradient SDSPAGE gels (BioRad, 567–8094) and visualized using ImageLabTM (BioRad) to detect proteolytic activity on POL A. .. Band intensities were measured with ImageLabTM (BioRad) and calculations were made in order to provide % remaining POL A-values.

Control:

Article Title: Promiscuity of response regulators for thioredoxin steers bacterial virulence.
Article Snippet: 6His-tag TrxA proteins were detected by immunoblot using a 1:1000 dilution of an anti-6His antibody (cat. # 600-401-382, Rockland Immunochemicals, Limerick, PA), followed by a 1:10,000 dilution of horseradish peroxidase-conjugated, goat anti-rabbit IgG antibodies (Fisher Scientific). .. As an internal control, 10μl of cell-free extracts were loaded onto 4-20% gradient SDSPAGE gels (cat. # 5671094, Bio-Rad, Hercules, CA). .. The blots were probed with 1: 5000 of an anti-DnaK monoclonal antibody (cat. # ab69617, Abcam, Waltham, MA), followed by the addition of 1:20,000 of a goat anti-mouse IgG conjugated with horseradish peroxidase (Fisher Scientific).